GLucocorticoids inhibit the generation of leukocyte procoagulant (tissue factor) activity

GLucocorticoids inhibit the generation of leukocyte procoagulant (tissue factor) activity.

Soluble guanylate cyclase agonists inhibit expression and procoagulant activity of tissue factor.

OBJECTIVE Tissue factor (TF), a major initiator of blood coagulation, contributes to inflammation, atherosclerosis, angiogenesis, and vascular remodeling. Pharmacological agonists of soluble guanylate cyclase (sGC) attenuate systemic and pulmonary hypertension, vascular remodeling, and platelet aggregation. However, the influence of these novel pharmacophores on TF is unknown. METHODS AND RES...

Cell Biology/Signaling Soluble Guanylate Cyclase Agonists Inhibit Expression and Procoagulant Activity of Tissue Factor

Objective—Tissue factor (TF), a major initiator of blood coagulation, contributes to inflammation, atherosclerosis, angiogenesis, and vascular remodeling. Pharmacological agonists of soluble guanylate cyclase (sGC) attenuate systemic and pulmonary hypertension, vascular remodeling, and platelet aggregation. However, the influence of these novel pharmacophores on TF is unknown. Methods and Resul...

Destruction of factor VIII procoagulant activity in tissue culture media.

Human endothelial cells have been repoded to synthesize protein that has von Willebrand factor activity and reacts with a heterologous antibody to plasma factor VIll/von Willebrand factor ( FVlll/vWF); however, no FVlll procoagulant activity has been associated with the protein produced by endothelial cells. Of its three biologic properties, only the FVlll procoagulant activity of purified plas...

Expression of tissue factor procoagulant activity: regulation by cytosolic calcium.

Intact bovine fibroblasts, pericytes, and kidney cells manifested significantly less tissue factor procoagulant activity than their disrupted counterparts. Addition of calcium ionophore A23187 rapidly and reversibly enhanced the cell-surface expression of tissue factor in intact cells up to the level achieved by disruption. Inhibitors of calmodulin blocked the ionophore-dependent enhancement of...